A high-copy T7 Escherichia coli expression vector for the production of recombinant proteins with a minimal N-terminal His-tagged fusion peptide

R. Ramos, Celso; Antônia Estima Abreu, Patrícia; L. T. O. Nascimento, Ana; Lee Ho, Paulo

doi:10.1590/s0100-879x2004000800001

Public

A high-copy T7 Escherichia coli expression vector for the production of recombinant proteins with a minimal N-terminal His-tagged fusion peptide

Published in Brazilian Journal of Medical and Biological Research • Aug 1, 2004

Authors:

Celso R. Ramos

Patrícia Antônia Estima Abreu

Ana L. T. O. Nascimento

Abstract

We report here the construction of a vector derived from pET3-His and pRSET plasmids for the expression and purification of recombinant proteins in Escherichia coli based on T7 phage RNA polymerase. The resulting pAE plasmid combined the advantages of both vectors: small size (pRSET), expression of ...

Subject

Subcloning

Plasmid

Recombinant DNA

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