Design and Evaluation of Useful Bacterium-Specific PCR Primers That Amplify Genes Coding for Bacterial 16S rRNA

R. Marchesi, Julian; Sato, Takuichi; J. Weightman, Andrew; A. Martin, Tracey; C. Fry, John; Hiom, Sarah; G. Wade, William

doi:10.1128/aem.64.2.795-799.1998

Public

Design and Evaluation of Useful Bacterium-Specific PCR Primers That Amplify Genes Coding for Bacterial 16S rRNA

Published in Applied and Environmental Microbiology • Feb 1, 1998

Authors:

Julian R. Marchesi

Takuichi Sato

Andrew J. Weightman

Abstract

We report the design and evaluation of PCR primers 63f and 1387r for amplification of 16S rRNA genes from bacteria. Their specificity and efficacy were tested systematically with a bacterial species and environmental samples. They were found to be more useful for 16S rRNA gene amplification in ecolo...

Subject

Biology

16S ribosomal RNA

Gene

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