Multiplex PCR for detection of plasmid-mediated quinolone resistance qnr genes in ESBL-producing enterobacterial isolates

Cattoir, Vincent; Poirel, Laurent; O. Rotimi, Vincent; Soussy, Claude-James; Nordmann, Patrice

doi:10.1093/jac/dkm204

Public

Multiplex PCR for detection of plasmid-mediated quinolone resistance qnr genes in ESBL-producing enterobacterial isolates

Published in Journal of Antimicrobial Chemotherapy • Jun 8, 2007

Authors:

Vincent Cattoir

Laurent Poirel

Vincent O. Rotimi

Abstract

OBJECTIVES: To develop a rapid and reliable single-tube-based PCR technique for detecting simultaneously the plasmid-mediated quinolone resistance qnrA, qnrB and qnrS genes. METHODS: After multiple alignments, primers were designed to detect known qnr variants (six for qnrA-, six for qnrB- and two f...

Subject

Quinolone

Plasmid

Multiplex polymerase chain reaction

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