Validation of reference genes for quantitative expression analysis by real-time RT-PCR in Saccharomyces cerevisiae

Teste, Marie-Ange; Duquenne, Manon; François, Jean; Parrou, Jean‐Luc

doi:10.1186/1471-2199-10-99

Public

Validation of reference genes for quantitative expression analysis by real-time RT-PCR in Saccharomyces cerevisiae

Published in BMC Molecular Biology • Oct 30, 2009

Authors:

Marie-Ange Teste

Manon Duquenne

Jean François

Abstract

BACKGROUND: Real-time RT-PCR is the recommended method for quantitative gene expression analysis. A compulsory step is the selection of good reference genes for normalization. A few genes often referred to as HouseKeeping Genes (HSK), such as ACT1, RDN18 or PDA1 are among the most commonly used, as ...

Subject

Reference genes

Housekeeping gene

Biology

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