Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications

Zipper, Herbert

doi:10.1093/nar/gnh101

Public

Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications

Published in Nucleic Acids Research • Jul 7, 2004

Authors:

Herbert Zipper

Abstract

The detection of double-stranded (ds) DNA by SYBR Green I (SG) is important in many molecular biology methods including gel electrophoresis, dsDNA quantification in solution and real-time PCR. Biophysical studies at defined dye/base pair ratios (dbprs) were used to determine the structure-property r...

Subject

Intercalation (chemistry)

Fluorescence

DNA

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