Culture of Human Endothelial Cells Derived from Umbilical Veins. IDENTIFICATION BY MORPHOLOGIC AND IMMUNOLOGIC CRITERIA

Jaffe, Eric; L. Nachman, Ralph; G. Becker, Carl; R Minick, C

doi:10.1172/jci107470

Public

Culture of Human Endothelial Cells Derived from Umbilical Veins. IDENTIFICATION BY MORPHOLOGIC AND IMMUNOLOGIC CRITERIA

Published in Journal of Clinical Investigation • Nov 1, 1973

Authors:

Eric Jaffe

Ralph L. Nachman

Carl G. Becker

Abstract

Endothelial cells were isolated from freshly obtained human umbilical cords by collagenase digestion of the interior of the umbilical vein. The cells were grown in tissue culture as a homogeneous population for periods up to 5 mo and some lines were subcultured for 10 serial passages. During the log...

Subject

Umbilical vein

Biology

Endothelial stem cell

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