Typing of rhizobia by PCR DNA fingerprinting and PCR-restriction fragment length polymorphism analysis of chromosomal and symbiotic gene regions: application to Rhizobium leguminosarum and its different biovars

Laguerre, Gisèle; Mavingui, Patrick; R Allard, M; Charnay, Marie‐Paule; Louvrier, Philippe; Mazurier, Sylvie; Rigottier‐Gois, Lionel; Amarger, N.

doi:10.1128/aem.62.6.2029-2036.1996

Public

Typing of rhizobia by PCR DNA fingerprinting and PCR-restriction fragment length polymorphism analysis of chromosomal and symbiotic gene regions: application to Rhizobium leguminosarum and its different biovars

Published in Applied and Environmental Microbiology • Jun 1, 1996

Authors:

Gisèle Laguerre

Patrick Mavingui

M R Allard

Abstract

Characterization of 43 strains of Rhizobium leguminosarum biovars viciae, trifolii, and phaseoli was performed by two methodologies based on PCR amplification, i.e., PCR DNA fingerprinting of interrepeat sequences and restriction fragment length polymorphism (RFLP) analysis of PCR -amplified chromos...

Subject

Biology

Restriction fragment length polymorphism

Biovar

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