Fluorometric Deoxyribonucleic Acid-Deoxyribonucleic Acid Hybridization in Microdilution Wells as an Alternative to Membrane Filter Hybridization in which Radioisotopes Are Used To Determine Genetic Relatedness among Bacterial Strains

EZAKI, T.; Hashimoto, Yohey; Yabuuchi, Eiko

doi:10.1099/00207713-39-3-224

Public

Fluorometric Deoxyribonucleic Acid-Deoxyribonucleic Acid Hybridization in Microdilution Wells as an Alternative to Membrane Filter Hybridization in which Radioisotopes Are Used To Determine Genetic Relatedness among Bacterial Strains

Published in International Journal of Systematic Bacteriology • Jul 1, 1989

Authors:

T. EZAKI

Yohey Hashimoto

Eiko Yabuuchi

Abstract

Fluorometric hybridization in microdilution wells was developed to determine genetic relatedness among microorganisms. Total chromosomal deoxyribonucleic acid (DNA) for hybridization reactions was labeled with photoreactive biotin (photobiotin). The biotinylated DNA was hybridized with single-strand...

Subject

Biotinylation

DNA

DNA–DNA hybridization

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