Influence of DNA Extraction Method, 16S rRNA Targeted Hypervariable Regions, and Sample Origin on Microbial Diversity Detected by 454 Pyrosequencing in Marine Chemosynthetic Ecosystems

Cruaud, Perrine; Vigneron, Adrien; Lucchetti-Miganeh, Céline; Emmanuel Ciron, Pierre; Godfroy, Anne; Cambon‐Bonavita, Marie‐Anne

doi:10.1128/aem.00592-14

Public

Influence of DNA Extraction Method, 16S rRNA Targeted Hypervariable Regions, and Sample Origin on Microbial Diversity Detected by 454 Pyrosequencing in Marine Chemosynthetic Ecosystems

Published in Applied and Environmental Microbiology • May 17, 2014

Authors:

Perrine Cruaud

Adrien Vigneron

Céline Lucchetti-Miganeh

Abstract

Next-generation sequencing (NGS) opens up exciting possibilities for improving our knowledge of environmental microbial diversity, allowing rapid and cost-effective identification of both cultivated and uncultivated microorganisms. However, library preparation, sequencing, and analysis of the result...

Subject

Pyrosequencing

Biology

Hypervariable region

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