Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms

Gregory Caporaso, J.; L. Lauber, Christian; A. Walters, William; Berg-Lyons, Donna; S. Huntley, James; Fierer, Noah; M. Owens, Sarah; Betley, Jason; Fraser, Louise; Bauer, Markus; Gormley, Niall; A. Gilbert, Jack; Smith, Geoff; Knight, Rob

doi:10.1038/ismej.2012.8

Public

Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms

Published in The ISME Journal • Mar 8, 2012

Authors:

J. Gregory Caporaso

Christian L. Lauber

William A. Walters

Abstract

DNA sequencing continues to decrease in cost with the Illumina HiSeq2000 generating up to 600 Gb of paired-end 100 base reads in a ten-day run. Here we present a protocol for community amplicon sequencing on the HiSeq2000 and MiSeq Illumina platforms, and apply that protocol to sequence 24 microbial...

Subject

Biology

Amplicon sequencing

Amplicon

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