Type II Toxoplasma gondii<i>KU80</i>Knockout Strains Enable Functional Analysis of Genes Required for Cyst Development and Latent Infection

A. Fox, Barbara; Falla, Alejandra; M. Rommereim, Leah; Tomita, Tadakimi; P. Gigley, Jason; Mercier, Corinne; Cesbron-Delauw, Marie-France; M. Weiss, Louis; J. Bzik, David

doi:10.1128/ec.00297-10

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Type II Toxoplasma gondiiKU80Knockout Strains Enable Functional Analysis of Genes Required for Cyst Development and Latent Infection

Shared by NobleBlocks on Sep 1, 2011 • 12:00 AM UTC

Authors:

Barbara A. Fox

Alejandra Falla

Leah M. Rommereim

Abstract

ABSTRACT Type II Toxoplasma gondii KU80 knockouts (Δ ku80 ) deficient in nonhomologous end joining were developed to delete the dominant pathway mediating random integration of targeting episomes. Gene targeting frequency in the type II Δ ku80 Δ hxgprt strain measured at the orotate ( OPRT ) and the...

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