Detection of Shiga-Like Toxin ( stx 1 and stx 2 ), Intimin ( eaeA ), and Enterohemorrhagic Escherichia coli (EHEC) Hemolysin (EHEC hlyA ) Genes in Animal Feces by Multiplex PCR

K. Fagan, Peter; Hornitzky, M.; A. Bettelheim, K.; P. Djordjevic, Steven

doi:10.1128/aem.65.2.868-872.1999

Public

Detection of Shiga-Like Toxin ( stx 1 and stx 2 ), Intimin ( eaeA ), and Enterohemorrhagic Escherichia coli (EHEC) Hemolysin (EHEC hlyA ) Genes in Animal Feces by Multiplex PCR

Published in Applied and Environmental Microbiology • Feb 1, 1999

Authors:

Peter K. Fagan

M. Hornitzky

K. A. Bettelheim

Abstract

A multiplex PCR was developed for the rapid detection of genes encoding Shiga toxins 1 and 2 (stx1 and stx2), intimin (eaeA), and enterohemolysin A (hlyA) in 444 fecal samples derived from healthy and clinically affected cattle, sheep, pigs, and goats. The method involved non-solvent-based extractio...

Subject

Intimin

Shiga toxin

Microbiology

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