The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments

A. Bustin, Stephen; Beneš, Vladimı́r; A. Garson, Jeremy; Hellemans, Jan; F. Huggett, Jim; Kubista, Mikael; Mueller, Reinhold; Nolan, Tania; W. Pfaffl, Michael; L. Shipley, Gregory; Vandesompele, Jo; T. Wittwer, Carl

doi:10.1373/clinchem.2008.112797

Public

The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments

Published in Clinical Chemistry • Feb 27, 2009

Authors:

Stephen A. Bustin

Vladimı́r Beneš

Jeremy A. Garson

Abstract

BACKGROUND: Currently, a lack of consensus exists on how best to perform and interpret quantitative real-time PCR (qPCR) experiments. The problem is exacerbated by a lack of sufficient experimental detail in many publications, which impedes a reader's ability to evaluate critically the quality of th...

Subject

Real-time polymerase chain reaction

Computer science

Computational biology

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