A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

Sikorski, R; Hieter, P.

doi:10.1093/genetics/122.1.19

Public

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

Published in Genetics • May 1, 1989

Authors:

R Sikorski

P. Hieter

Abstract

A series of yeast shuttle vectors and host strains has been created to allow more efficient manipulation of DNA in Saccharomyces cerevisiae. Transplacement vectors were constructed and used to derive yeast strains containing nonreverting his3, trp1, leu2 and ura3 mutations. A set of YCp and YIp vect...

Subject

URA3

Shuttle vector

Biology

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