Genetic heterogeneity of ribosomal internal transcribed spacer in clinical samples of Leishmania donovani spotted on filter paper as revealed by single-strand conformation polymorphisms and sequencing

O. El Tai, Nahla; F. Osman, O.; El Fari, Mustafa; Presber, W; Schönian, Gabriele

doi:10.1016/s0035-9203(00)90093-2

Public

Genetic heterogeneity of ribosomal internal transcribed spacer in clinical samples of Leishmania donovani spotted on filter paper as revealed by single-strand conformation polymorphisms and sequencing

Published in Transactions of the Royal Society of Tropical Medicine and Hygiene • Sep 1, 2000

Authors:

Nahla O. El Tai

O. F. Osman

Mustafa El Fari

Abstract

A polymerase chain reaction and single-strand conformation polymorphism determination (PCR-SSCP) was used to detect deoxyribonucleic acid sequence polymorphisms in the transcribed non-coding regions between the small and large sub-unit ribosomal ribonucleic acid (rRNA) genes in Leishmania donovani f...

Subject

Internal transcribed spacer

Single-strand conformation polymorphism

Biology

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