One-Step Sequence- and Ligation-Independent Cloning as a Rapid and Versatile Cloning Method for Functional Genomics Studies

Jeong, Jae-Yeon; Yim, Hyung‐Soon; Ryu, Ji-Young; Sook Lee, Hyun; Lee, Jung-Hyun; Seen, Dong‐Seung; Gyun Kang, Sung

doi:10.1128/aem.00844-12

Public

One-Step Sequence- and Ligation-Independent Cloning as a Rapid and Versatile Cloning Method for Functional Genomics Studies

Published in Applied and Environmental Microbiology • May 20, 2012

Authors:

Jae-Yeon Jeong

Hyung‐Soon Yim

Ji-Young Ryu

Abstract

We developed one-step sequence- and ligation-independent cloning (SLIC) as a simple, cost-effective, time-saving, and versatile cloning method. Highly efficient and directional cloning can be achieved by direct bacterial transformation 2.5 min after mixing any linearized vector, an insert(s) prepare...

Subject

Cloning (programming)

Transformation (genetics)

Cloning vector

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