Use of an Allele-Specific Polymerase Chain Reaction Assay to Genotype Pyrethroid Resistant Strains of<i>Boophilus microplus</i>(Acari: Ixodidae)

D. Guerrero, Felix; B. Davey, Ronald; J. Miller, Robert

doi:10.1603/0022-2585-38.1.44

Public

Use of an Allele-Specific Polymerase Chain Reaction Assay to Genotype Pyrethroid Resistant Strains ofBoophilus microplus(Acari: Ixodidae)

Published in Journal of Medical Entomology • Jan 1, 2001

Authors:

Felix D. Guerrero

Ronald B. Davey

Robert J. Miller

Abstract

A polymerase chain reaction-based assay was developed to detect the presence of a pyrethroid resistance-associated amino acid substitution in Boophilus microplus (Canestrini). The assay uses a simple method for the extraction of genomic DNA from individual larvae and genotypes individuals for the pr...

Subject

Biology

Pyrethroid

Ixodidae

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