Isolation of Exosomes from Blood Plasma: Qualitative and Quantitative Comparison of Ultracentrifugation and Size Exclusion Chromatography Methods

Baranyai, Tamás; Herczeg, Kata; Onódi, Zsófia; Voszka, István; Módos, Károly; Marton, Nikolett; Nagy, György; Mäger, Imre; J. A. Wood, Matthew; EL Andaloussi, Samir; Pálinkás, Zoltán; Kumar, Vikas; Nagy, Péter; Kittel, Ágnes; I. Buzás, Edit; Ferdinandy, Péter; Giricz, Zoltán

doi:10.1371/journal.pone.0145686

Public

Isolation of Exosomes from Blood Plasma: Qualitative and Quantitative Comparison of Ultracentrifugation and Size Exclusion Chromatography Methods

Published in PLoS ONE • Dec 21, 2015

Authors:

Tamás Baranyai

Kata Herczeg

Zsófia Onódi

Abstract

BACKGROUND: Exosomes are emerging targets for biomedical research. However, suitable methods for the isolation of blood plasma-derived exosomes without impurities have not yet been described. AIM: Therefore, we investigated the efficiency and purity of exosomes isolated with potentially suitable met...

Subject

Microvesicles

Albumin

Nanoparticle tracking analysis

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