One fungus, which genes? Development and assessment of universal primers for potential secondary fungal DNA barcodes

Benjamin Stielow, J.; André Lévesque, C.; A. Seifert, Keith; Meyer, Wieland; Irinyi, László; J. Smits, Daphne; Renfurm, R.; Verkley, G.J.M.; Groenewald, Marizeth; Chaduli, Delphine; Lomascolo, Anne; Welti, Stéphane; Lesage‐Meessen, Laurence; Favel, Anne; M. S. Al‐Hatmi, Abdullah; Damm, Ulrike; Yılmaz, Neriman; Houbraken, Jos; Lombard, L.; Quaedvlieg, William; Binder, M.; Vaas, Lea; Vu, Duong; Yurkov, Andrey; Begerow, Dominik; Roehl, O.; Alexandre Guerreiro, Marco; Fonseca, Álvaro; Samerpitak, Kittipan; D. van Diepeningen, Anne; Dolatabadi, Somayeh; F. Moreno, Leandro; Casarégola, Serge; Mallet, Sandrine; Jacques, Noémie; Roscini, Luca; Egidi, Eleonora; Bizet, Chantal; Garcia‐Hermoso, Dea; P. Martín, María; Deng, Siwen; Groenewald, J.Z.; Boekhout, Teun; Wilhelm de Beer, Z.; Barnes, Irene; A. Duong, Tuan; J. Wingfield, Michael; de Hoog, Sybren; Crous, P.W.; T. Lewis, Christopher; Hambleton, Sarah; A. A. Moussa, Tarek; Alzahrani, Hassan; A. Almaghrabi, Omar; Louis-Seize, Gerry; Assabgui, R.; McCormick, Wayne; Omer, G.; Dukik, Karolina; Cardinali, Gianluigi; Eberhardt, Ursula; de Vries, Michèl; Robert, Vincent

doi:10.3767/003158515x689135

Public

One fungus, which genes? Development and assessment of universal primers for potential secondary fungal DNA barcodes

Published in Persoonia - Molecular Phylogeny and Evolution of Fungi • Aug 31, 2015

Authors:

J. Benjamin Stielow

C. André Lévesque

Keith A. Seifert

Abstract

The aim of this study was to assess potential candidate gene regions and corresponding universal primer pairs as secondary DNA barcodes for the fungal kingdom, additional to ITS rDNA as primary barcode. Amplification efficiencies of 14 (partially) universal primer pairs targeting eight genetic marke...

Subject

Biology

Primer (cosmetics)

Genetics

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