MiFish, a set of universal PCR primers for metabarcoding environmental DNA from fishes: detection of more than 230 subtropical marine species

Miya, Masaki; Sato, Yukuto; Fukunaga, Tsukasa; Sado, Tetsuya; Yde Poulsen, Jan; Sato, Keiichi; Minamoto, Toshifumi; Yamamoto, Satoshi; Yamanaka, Hiroki; Araki, Hitoshi; Kondoh, Michio; Iwasaki, Wataru

doi:10.1098/rsos.150088

Public

MiFish, a set of universal PCR primers for metabarcoding environmental DNA from fishes: detection of more than 230 subtropical marine species

Published in Royal Society Open Science • Jul 1, 2015

Authors:

Masaki Miya

Yukuto Sato

Tsukasa Fukunaga

Abstract

We developed a set of universal PCR primers (MiFish-U/E) for metabarcoding environmental DNA (eDNA) from fishes. Primers were designed using aligned whole mitochondrial genome (mitogenome) sequences from 880 species, supplemented by partial mitogenome sequences from 160 elasmobranchs (sharks and ray...

Subject

Environmental DNA

Biology

Pelagic zone

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