Absolute and direct microRNA quantification using DNA-gold nanoparticle probes.

Degliangeli, Federica; Kshirsagar, Prakash; Brunetti, Virgilio; Paolo Pompa, Pier; Fiammengo, Roberto

doi:10.1021/ja412152x

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Absolute and direct microRNA quantification using DNA-gold nanoparticle probes.

Shared by NobleBlocks on Feb 12, 2014 • 12:00 AM UTC

Authors:

Federica Degliangeli

Prakash Kshirsagar

Virgilio Brunetti

Abstract

DNA-gold nanoparticle probes are implemented in a simple strategy for direct microRNA (miRNA) quantification. Fluorescently labeled DNA-probe strands are immobilized on PEGylated gold nanoparticles (AuNPs). In the presence of target miRNA, DNA-RNA heteroduplexes are formed and become substrate for t...

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