Dissecting the multicellular ecosystem of metastatic melanoma by single-cell RNA-seq

Tirosh, Itay; Izar, Benjamin; M. Prakadan, Sanjay; H. Wadsworth, Marc; J. Treacy, Daniel; J. Trombetta, John; Rotem, Asaf; Rodman, Christopher; G. Lian, Christine; F. Murphy, Gëorge; Fallahi‐Sichani, Mohammad; Dutton‐Regester, Ken; Lin, Jia‐Ren; Cohen, Ofir; Shah, Parin; Lu, Diana; S. Genshaft, Alex; K. Hughes, Travis; G.K. Ziegler, Carly; W. Kazer, Samuel; Gaillard, Aleth; E. Kolb, Kellie; Villani, Alexandra–Chloé; M. Johannessen, Cory; Andreev, Aleksandr; M. Van Allen, Eliezer; M. Bertagnolli, Monica; K. Sorger, Peter; J. Sullivan, Ryan; T. Flaherty, Keith; T. Frederick, Dennie; Jané‐Valbuena, Judit; H. Yoon, Charles; Rozenblatt–Rosen, Orit; K. Shalek, Alex; Regev, Aviv; A. Garraway, Levi

doi:10.1126/science.aad0501

Public

Dissecting the multicellular ecosystem of metastatic melanoma by single-cell RNA-seq

Published in Science • Apr 7, 2016

Authors:

Itay Tirosh

Benjamin Izar

Sanjay M. Prakadan

Abstract

To explore the distinct genotypic and phenotypic states of melanoma tumors, we applied single-cell RNA sequencing (RNA-seq) to 4645 single cells isolated from 19 patients, profiling malignant, immune, stromal, and endothelial cells. Malignant cells within the same tumor displayed transcriptional het...

Subject

Microphthalmia-associated transcription factor

Biology

Melanoma

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