CEL-Seq2: sensitive highly-multiplexed single-cell RNA-Seq

Hashimshony, Tamar; Senderovich, Naftalie; Avital, Gal; Klochendler, Agnes; de Leeuw, Yaron; Anavy, Leon; Gennert, Dave; Li, Shuqiang; J. Livak, Kenneth; Rozenblatt–Rosen, Orit; Dor, Yuval; Regev, Aviv; Yanai, Itai

doi:10.1186/s13059-016-0938-8

Public

CEL-Seq2: sensitive highly-multiplexed single-cell RNA-Seq

Published in Genome biology • Apr 27, 2016

Authors:

Tamar Hashimshony

Naftalie Senderovich

Gal Avital

Abstract

Single-cell transcriptomics requires a method that is sensitive, accurate, and reproducible. Here, we present CEL-Seq2, a modified version of our CEL-Seq method, with threefold higher sensitivity, lower costs, and less hands-on time. We implemented CEL-Seq2 on Fluidigm's C1 system, providing its fir...

Subject

Biology

RNA-Seq

Computational biology

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