Structure of fully protonated proteins by proton-detected magic-angle spinning NMR

B. Andreas, Loren; Jaudzems, Kristaps; Staněk, Jan; Lalli, Daniela; Bertarello, Andrea; Le Marchand, Tanguy; Cala-De Paepe, D.; Koteloviča, Svetlana; Akopjana, Ināra; Knott, Benno; Wegner, Sebastian; Engelke, Frank; Lesage, Anne; Emsley, Lyndon; Tārs, Kaspars; Herrmann, Torsten; Pintacuda, Guido

doi:10.1073/pnas.1602248113

Public

Structure of fully protonated proteins by proton-detected magic-angle spinning NMR

Published in Proceedings of the National Academy of Sciences • Aug 3, 2016

Authors:

Loren B. Andreas

Kristaps Jaudzems

Jan Staněk

Abstract

Protein structure determination by proton-detected magic-angle spinning (MAS) NMR has focused on highly deuterated samples, in which only a small number of protons are introduced and observation of signals from side chains is extremely limited. Here, we show in two fully protonated proteins that, at...

Subject

Magic angle spinning

Spins

Protonation

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