smiFISH and FISH-quant – a flexible single RNA detection approach with super-resolution capability

Tsanov, Nikolay; Samacoïts, Aubin; Chouaib, Racha; Traboulsi, Abdel-Meneem; Gostan, Thierry; Weber, Christian; Zimmer, Christophe; Zibara, Kazem; Walter, Thomas; Peter, Marion; Bertrand, Édouard; Mueller, Florian

doi:10.1093/nar/gkw784

Public

smiFISH and FISH-quant – a flexible single RNA detection approach with super-resolution capability

Published in Nucleic Acids Research • Sep 5, 2016

Authors:

Nikolay Tsanov

Aubin Samacoïts

Racha Chouaib

Abstract

Single molecule FISH (smFISH) allows studying transcription and RNA localization by imaging individual mRNAs in single cells. We present smiFISH (single molecule inexpensive FISH), an easy to use and flexible RNA visualization and quantification approach that uses unlabelled primary probes and a flu...

Subject

Biology

Oligonucleotide

RNA

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