Compact and highly active next-generation libraries for CRISPR-mediated gene repression and activation

A Horlbeck, Max; A Gilbert, Luke; E Villalta, Jacqueline; Adamson, Britt; A Pak, Ryan; Chen, Yuwen; P Fields, Alexander; Yon Park, Chong; E Corn, Jacob; Kampmann, Martin; S Weissman, Jonathan

doi:10.7554/elife.19760

Public

Compact and highly active next-generation libraries for CRISPR-mediated gene repression and activation

Shared by NobleBlocks on Sep 23, 2016 • 12:00 AM UTC

Authors:

Max A Horlbeck

Luke A Gilbert

Jacqueline E Villalta

Abstract

We recently found that nucleosomes directly block access of CRISPR/Cas9 to DNA (<xref ref-type="bibr" rid="bib25">Horlbeck et al., 2016</xref>). Here, we build on this observation with a comprehensive algorithm that incorporates chromatin, position, and sequence features to accurately predict highly...

Discussions