A Comparative Study of Serum Exosome Isolation Using Differential Ultracentrifugation and Three Commercial Reagents

Helwa, Inas; Cai, Jingwen; Drewry, Michelle; D. Zimmerman, Arthur; B. Dinkins, Michael; Lotfy Khaled, Mariam; Seremwe, Mutsa; Michael Dismuke, W.; Bieberich, Erhard; Daniel Stamer, W.; W. Hamrick, Mark; Liu, Yutao

doi:10.1371/journal.pone.0170628

Public

A Comparative Study of Serum Exosome Isolation Using Differential Ultracentrifugation and Three Commercial Reagents

Published in PLoS ONE • Jan 23, 2017

Authors:

Inas Helwa

Jingwen Cai

Michelle Drewry

Abstract

Exosomes play a role in cell-to-cell signaling and serve as possible biomarkers. Isolating exosomes with reliable quality and substantial concentration is a major challenge. Our purpose is to compare the exosomes extracted by three different exosome isolation kits (miRCURY, ExoQuick, and Invitrogen ...

Subject

Ultracentrifuge

Analytical Ultracentrifugation

Exosome

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