Microfluidic droplet platform for ultrahigh-throughput single-cell screening of biodiversity

S. Terekhov, Stanislav; V. Smirnov, Ivan; V. Stepanova, Anastasiya; V. Bobik, Tatyana; A. Mokrushina, Yuliana; A. Ponomarenko, Natalia; A. Belogurov, Alexey; P. Rubtsova, Maria; V. Kartseva, Olga; O. Gomzikova, Marina; A. Moskovtsev, Alexey; S. Bukatin, Anton; V. Dubina, Michael; S. Kostryukova, Elena; V. Babenko, Vladislav; T. Vakhitova, Maria; I. Manolov, Alexander; V. Malakhova, Maja; A. Kornienko, Maria; V. Tyakht, Alexander; A. Vanyushkina, Anna; N. Ilina, Elena; Masson, Patrick; G. Gabibov, Alexander; Altman, Sidney

doi:10.1073/pnas.1621226114

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Microfluidic droplet platform for ultrahigh-throughput single-cell screening of biodiversity

Shared by NobleBlocks on Feb 15, 2017 • 12:00 AM UTC

Authors:

Stanislav S. Terekhov

Ivan V. Smirnov

Anastasiya V. Stepanova

Abstract

Significance Biocompatible microfluidic double water-in-oil-in-water emulsion (MDE) enables in-droplet cultivation of different living species. The combination of droplet-generating machinery with FACS followed by next-generation sequencing and liquid chromatography-mass spectrometry analysis of the...

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