Comparative Evaluation of Four Bacteria-Specific Primer Pairs for 16S rRNA Gene Surveys

Thijs, Sofie; Op De Beeck, Michiel; Beckers, Bram; Truyens, Sascha; Stevens, Vincent; D. Van Hamme, Jonathan; Weyens, Nele; Vangronsveld, Jaco

doi:10.3389/fmicb.2017.00494

Public

Comparative Evaluation of Four Bacteria-Specific Primer Pairs for 16S rRNA Gene Surveys

Published in Frontiers in Microbiology • Mar 28, 2017

Authors:

Sofie Thijs

Michiel Op De Beeck

Bram Beckers

Abstract

Bacterial taxonomic community analyses using PCR-amplification of the 16S rRNA gene and high-throughput sequencing has become a cornerstone in microbiology research. To reliably detect the members, or operational taxonomic units (OTUs), that make up bacterial communities, taxonomic surveys rely on t...

Subject

Biology

16S ribosomal RNA

Primer (cosmetics)

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