Methods to determine limit of detection and limit of quantification in quantitative real-time PCR (qPCR)

Forootan, Amin; Sjöback, Robert; Björkman, Jens; Sjögreen, Björn; B. Linz, Lucas; Kubista, Mikael

doi:10.1016/j.bdq.2017.04.001

Public

Methods to determine limit of detection and limit of quantification in quantitative real-time PCR (qPCR)

Published in Biomolecular Detection and Quantification • Apr 29, 2017

Authors:

Amin Forootan

Robert Sjöback

Jens Björkman

Abstract

Quantitative Real-Time Polymerase Chain Reaction, better known as qPCR, is the most sensitive and specific technique we have for the detection of nucleic acids. Even though it has been around for more than 30 years and is preferred in research applications, it has yet to win broad acceptance in rout...

Subject

Detection limit

Real-time polymerase chain reaction

Limit (mathematics)

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