Easi-CRISPR: a robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins

M. Quadros, Rolen; Miura, Hiromi; W. Harms, Donald; Akatsuka, Hisako; Sato, Takehito; Aida, Tomomi; Redder, Ronald; P. Richardson, Guy; Inagaki, Yutaka; Sakai, Daisuke; M. Buckley, Shannon; Seshacharyulu, Parthasarathy; K. Batra, Surinder; A. Behlke, Mark; A. Zeiner, Sarah; M. Jacobi, Ashley; Izu, Yayoi; B. Thoreson, Wallace; D. Urness, Lisa; L. Mansour, Suzanne; Ohtsuka, Masato; B. Gurumurthy, Channabasavaiah

doi:10.1186/s13059-017-1220-4

Public

Easi-CRISPR: a robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins

Shared by NobleBlocks on May 17, 2017 • 12:00 AM UTC

Authors:

Rolen M. Quadros

Hiromi Miura

Donald W. Harms

Abstract

Abstract Background Conditional knockout mice and transgenic mice expressing recombinases, reporters, and inducible transcriptional activators are key for many genetic studies and comprise over 90% of mouse models created. Conditional knockout mice are generated using labor-intensive methods of homo...

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