Rapid Proton-Detected NMR Assignment for Proteins with Fast Magic Angle Spinning

Barbet‐Massin, Emeline; J. Pell, Andrew; Sebastian Retel, Joren; B. Andreas, Loren; Jaudzems, Kristaps; Trent Franks, W.; J. Nieuwkoop, Andrew; Hiller, Matthias; Ann Higman, Victoria; Guerry, Paul; Bertarello, Andrea; J. Knight, Michael; Felletti, Michèle; Le Marchand, Tanguy; Koteloviča, Svetlana; Akopjana, Ināra; Tārs, Kaspars; Stoppini, Monica; Bellotti, Vittorio; Bolognesi, Martino; Ricagno, Stéfano; J. Chou, James; G. Griffin, Robert; Oschkinat, Hartmut; Lesage, Anne; Emsley, Lyndon; Herrmann, Torsten; Pintacuda, Guido

doi:10.1021/ja507382j

Public

Rapid Proton-Detected NMR Assignment for Proteins with Fast Magic Angle Spinning

Published in Journal of the American Chemical Society • Aug 7, 2014

Authors:

Emeline Barbet‐Massin

Andrew J. Pell

Joren Sebastian Retel

Abstract

Using a set of six (1)H-detected triple-resonance NMR experiments, we establish a method for sequence-specific backbone resonance assignment of magic angle spinning (MAS) nuclear magnetic resonance (NMR) spectra of 5-30 kDa proteins. The approach relies on perdeuteration, amide (2)H/(1)H exchange, h...

Subject

Chemistry

Magic angle spinning

Spinning

Finding related papers...

Discussions

(0)

No comments yet

Be the first to share your thoughts!