SLAM-seq defines direct gene-regulatory functions of the BRD4-MYC axis

Muhar, Matthias; Ebert, Anja; Neumann, Tobias; Umkehrer, Christian; Jude, Julian; Wieshofer, Corinna; Rescheneder, Philipp; Lipp, Jesse; A. Herzog, Veronika; Reichholf, Brian; A. Cisneros, David; Hoffmann, Thomas; F. Schlapansky, Moritz; Bhat, Pooja; von Haeseler, Arndt; Köcher, Thomas; C. Obenauf, Anna; Popow, Johannes; L. Ameres, Stefan; Zuber, Johannes

doi:10.1126/science.aao2793

Public

SLAM-seq defines direct gene-regulatory functions of the BRD4-MYC axis

Published in Science • Apr 5, 2018

Authors:

Matthias Muhar

Anja Ebert

Tobias Neumann

Abstract

Defining direct targets of transcription factors and regulatory pathways is key to understanding their roles in physiology and disease. We combined SLAM-seq [thiol(SH)-linked alkylation for the metabolic sequencing of RNA], a method for direct quantification of newly synthesized messenger RNAs (mRNA...

Subject

BRD4

Ribosome biogenesis

Bromodomain

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