A CRISPRi screen in E. coli reveals sequence-specific toxicity of dCas9

Abstract High-throughput CRISPR-Cas9 screens have recently emerged as powerful tools to decipher gene functions and genetic interactions. Here we use a genome-wide library of guide RNAs to direct the catalytically dead Cas9 (dCas9) to block gene transcription in Escherichia coli . Using a machine-le... View More