Systematic Methodological Evaluation of a Multiplex Bead-Based Flow Cytometry Assay for Detection of Extracellular Vesicle Surface Signatures

P. B. Wiklander, Oscar; Beklem Bostancıoğlu, Rakibe; A Welsh, Joshua; M. Zickler, Antje; Murke, Florian; Corso, Giulia; Felldin, Ulrika; W. Hagey, Daniel; Evertsson, Björn; Liang, Xiuming; O. Gustafsson, Manuela; K. Mohammad, Dara; Wiek, Constanze; Hanenberg, Helmut; Bremer, Michel; Gupta, Dhanu; Björnstedt, Mikael; Giebel, Bernd; Z. Nordin, Joel; Jones, Jennifer; EL Andaloussi, Samir; Görgens, André

doi:10.3389/fimmu.2018.01326

Public

Systematic Methodological Evaluation of a Multiplex Bead-Based Flow Cytometry Assay for Detection of Extracellular Vesicle Surface Signatures

Published in Frontiers in Immunology • Jun 13, 2018

Authors:

Oscar P. B. Wiklander

Rakibe Beklem Bostancıoğlu

Joshua A Welsh

Abstract

Extracellular vesicles (EVs) can be harvested from cell culture supernatants and from all body fluids. EVs can be conceptually classified based on their size and biogenesis as exosomes and microvesicles. Nowadays, it is however commonly accepted in the field that there is a much higher degree of het...

Subject

Extracellular vesicles

Multiplex

Flow cytometry

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