Activities and specificities of <scp>CRISPR</scp>/Cas9 and Cas12a nucleases for targeted mutagenesis in maize

Lee, Keunsub; Zhang, Yingxiao; P. Kleinstiver, Benjamin; A. Guo, Jimmy; J. Aryee, Martin; Miller, Jonah; A. Malzahn, Aimee; Zarecor, Scott; J. Lawrence‐Dill, Carolyn; Keith Joung, J.; Qi, Yiping; Wang, Kan

doi:10.1111/pbi.12982

Public

Activities and specificities of CRISPR/Cas9 and Cas12a nucleases for targeted mutagenesis in maize

Published in Plant Biotechnology Journal • Jul 4, 2018

Authors:

Keunsub Lee

Yingxiao Zhang

Benjamin P. Kleinstiver

Abstract

CRISPR/Cas9 and Cas12a (Cpf1) nucleases are two of the most powerful genome editing tools in plants. In this work, we compared their activities by targeting maize glossy2 gene coding region that has overlapping sequences recognized by both nucleases. We introduced constructs carrying SpCas9-guide RN...

Subject

CRISPR

Biology

Genome editing

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