CRISPR-Cas9 genome editing induces megabase-scale chromosomal truncations

Cullot, Grégoire; Boutin, Julian; Toutain, Jérôme; Prat, Florence; Pennamen, Perrine; Rooryck, Caroline; Teichmann, Martin; Rousseau, Emilie; Lamrissi‐Garcia, Isabelle; Guyonnet‐Dupérat, Veronique; Bibeyran, Alice; Lalanne, Magalie; Prouzet‐Mauleon, Valérie; Turcq, Béatrice; Ged, Cécile; Blouin, Jean‐Marc; Richard, Emmanuel; Dabernat, Sandrine; Moreau‐Gaudry, François; Bedel, Aurélie

doi:10.1038/s41467-019-09006-2

Public

CRISPR-Cas9 genome editing induces megabase-scale chromosomal truncations

Published in Nature Communications • Mar 8, 2019

Authors:

Grégoire Cullot

Julian Boutin

Jérôme Toutain

Abstract

CRISPR-Cas9 is a promising technology for genome editing. Here we use Cas9 nuclease-induced double-strand break DNA (DSB) at the UROS locus to model and correct congenital erythropoietic porphyria. We demonstrate that homology-directed repair is rare compared with NHEJ pathway leading to on-target i...

Subject

CRISPR

Genome editing

Cas9

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