Expression of recombinant enhanced green fluorescent protein provides insight into foreign gene‐expression differences between <scp>Mut+</scp> and <scp>MutS</scp> strains of <i>Pichia pastoris</i>

W. Theron, Chrispian; Berrios, Julio; Steels, Sébastien; Telek, Samuel; Lecler, Renaud; Rodriguez, Christian; Fickers, Patrick

doi:10.1002/yea.3388

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Expression of recombinant enhanced green fluorescent protein provides insight into foreign gene‐expression differences between Mut+ and MutS strains of Pichia pastoris

Shared by NobleBlocks on May 1, 2019 • 12:00 AM UTC

Authors:

Chrispian W. Theron

Julio Berrios

Sébastien Steels

Abstract

Abstract Pichia pastoris is a very popular yeast for recombinant protein production, mainly due to the strong, methanol‐inducible P AOX1 promoter. Methanol induction however poses several drawbacks. One approach to improve processes is to use MutS strains with reduced methanol catabolic ability. Var...

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