HOLMESv2: A CRISPR-Cas12b-Assisted Platform for Nucleic Acid Detection and DNA Methylation Quantitation

Li, Linxian; Li, Shiyuan; Wu, Na; Wu, Jiacheng; Wang, Gang; Zhao, Guoping; Wang, Jin

doi:10.1021/acssynbio.9b00209

Public

HOLMESv2: A CRISPR-Cas12b-Assisted Platform for Nucleic Acid Detection and DNA Methylation Quantitation

Published in ACS Synthetic Biology • Sep 18, 2019

Authors:

Linxian Li

Shiyuan Li

Na Wu

Abstract

-cleavage activity against collateral single-stranded DNA (ssDNA) and employed the activity to develop a rapid nucleic acid detection system, namely HOLMES (one-hour low-cost multipurpose highly efficient system). Here, with the employment of thermophilic CRISPR-Cas12b, we create HOLMESv2 for four d...

Subject

CRISPR

Nucleic acid

Computational biology

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