Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays

H. D. Crawford, Katharine; Eguia, Rachel; S. Dingens, Adam; N. Loes, Andrea; D. Malone, Keara; R. Wolf, Caitlin; Y. Chu, Helen; Alejandra Tortorici, M.; Veesler, David; Murphy, Michael; Pettie, Deleah; P. King, Neil; B. Balazs, Alejandro; D. Bloom, Jesse

doi:10.3390/v12050513

Public

Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays

Published in Viruses • May 6, 2020

Authors:

Katharine H. D. Crawford

Rachel Eguia

Adam S. Dingens

Abstract

SARS-CoV-2 enters cells using its Spike protein, which is also the main target of neutralizing antibodies. Therefore, assays to measure how antibodies and sera affect Spike-mediated viral infection are important for studying immunity. Because SARS-CoV-2 is a biosafety-level-3 virus, one way to simpl...

Subject

Virology

Neutralization

Vesicular stomatitis virus

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