A single-cell and single-nucleus RNA-Seq toolbox for fresh and frozen human tumors

Slyper, Michal; Porter, Caroline; Ashenberg, Orr; Waldman, Julia; Drokhlyansky, Eugene; Wakiro, Isaac; S. Smillie, Christopher; Smith-Rosario, Gabriela; Wu, Jingyi; Dionne, Danielle; Vigneau, Sébastien; Jané‐Valbuena, Judit; L. Tickle, Timothy; Napolitano, Sara; Su, Mei-Ju; G. Patel, Anand; Karlström, Åsa; Gritsch, Simon; Nomura, Masashi; Waghray, Avinash; H. Gohil, Satyen; M. Tsankov, Alexander; Jerby‐Arnon, Livnat; Cohen, Ofir; Klughammer, Johanna; Rosen, Yanay; Gould, Joshua; Nguyễn, Lan; Hofree, Matan; J. Tramontozzi, Peter; Li, Bo; J. Wu, Catherine; Izar, Benjamin; Haq, Rizwan; Stephen Hodi, F.; H. Yoon, Charles; N. Hata, Aaron; J. Baker, Suzanne; L. Suvà, Mario; Bueno, Raphael; H. Stover, Elizabeth; R. Clay, Michael; A. Dyer, Michael; B. Collins, Natalie; A. Matulonis, Ursula; Wagle, Nikhil; E. Johnson, Bruce; Rotem, Asaf; Rozenblatt–Rosen, Orit; Regev, Aviv

doi:10.1038/s41591-020-0844-1

Public

A single-cell and single-nucleus RNA-Seq toolbox for fresh and frozen human tumors

Published in Nature Medicine • May 1, 2020

Authors:

Michal Slyper

Caroline Porter

Orr Ashenberg

Abstract

Single-cell genomics is essential to chart tumor ecosystems. Although single-cell RNA-Seq (scRNA-Seq) profiles RNA from cells dissociated from fresh tumors, single-nucleus RNA-Seq (snRNA-Seq) is needed to profile frozen or hard-to-dissociate tumors. Each requires customization to different tissue an...

Subject

Toolbox

RNA

Small nuclear RNA

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