Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications

Datta, Rupsa; M. Heaster, Tiffany; T. Sharick, Joe; A. Gillette, Amani; C. Skala, Melissa

doi:10.1117/1.jbo.25.7.071203

Public

Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications

Published in Journal of Biomedical Optics • May 13, 2020

Authors:

Rupsa Datta

Tiffany M. Heaster

Joe T. Sharick

Abstract

SIGNIFICANCE: Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to distinguish the unique molecular environment of fluorophores. FLIM measures the time a fluorophore remains in an excited state before emitting a photon, and detects molecular variations of fluorophores that are ...

Subject

Fluorophore

Fluorescence-lifetime imaging microscopy

Förster resonance energy transfer

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