Topoisomerase 1 prevents replication stress at R-loop-enriched transcription termination sites

Promonet, Alexy; Padioleau, Ismaël; Liu, Yaqun; A. Sanz, Lionel; Biernacka, Anna; Schmitz, Anne-Lyne; Skrzypczak, Magdalena; Sarrazin, Amélie; Mettling, Clément; Rowicka, Maga; Ginalski, Krzysztof; Chédin, Frédéric; Chen, Chun-Long; Lin, Yea-Lih; Pasero, Philippe

doi:10.1038/s41467-020-17858-2

Public

Topoisomerase 1 prevents replication stress at R-loop-enriched transcription termination sites

Published in Nature Communications • Aug 7, 2020

Authors:

Alexy Promonet

Ismaël Padioleau

Yaqun Liu

Abstract

R-loops have both positive and negative impacts on chromosome functions. To identify toxic R-loops in the human genome, here, we map RNA:DNA hybrids, replication stress markers and DNA double-strand breaks (DSBs) in cells depleted for Topoisomerase I (Top1), an enzyme that relaxes DNA supercoiling a...

Subject

DNA supercoil

Topoisomerase

Biology

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