Dynamic single-cell phenotyping of immune cells using the microfluidic platform DropMap

Bounab, Yacine; Eyer, Klaus; Dixneuf, Sophie; Rybczyńska, Maria; Chauvel, Cécile; Mistretta, Maxime; Tran, Trang; Aymerich, Nathan; Chenon, Guilhem; Llitjos, Jean‐François; Venet, Fabienne; Monneret, Guillaume; Gillespie, Iain; Cortez, Pierre; Moucadel, Virginie; Pachot, Alexandre; Troesch, Alain; Leissner, Philippe; Textoris, Julien; Bibette, Jérôme; Guyard, Cyril; Baudry, Jean; D. Griffiths, Andrew; Védrine, Christophe

doi:10.1038/s41596-020-0354-0

Public

Dynamic single-cell phenotyping of immune cells using the microfluidic platform DropMap

Published in Nature Protocols • Aug 12, 2020

Authors:

Yacine Bounab

Klaus Eyer

Sophie Dixneuf

Abstract

Characterization of immune responses is currently hampered by the lack of systems enabling quantitative and dynamic phenotypic characterization of individual cells and, in particular, analysis of secreted proteins such as cytokines and antibodies. We recently developed a simple and robust microfluid...

Subject

ELISPOT

Flow cytometry

Single-cell analysis

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