Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results

Möller, Peter; Azqueta, Amaya; Boutet-Robinet, Élisa; Koppen, Gudrun; Bonassi, Stefano; Milić, Mirta; Gajski, Goran; Costa, Solange; Paulo Teixeira, João; Pereira, Cristiana; Dušinská, Mária; Godschalk, Roger; Brunborg, Gunnar; B. Gützkow, Kristine; Giovannelli, Lisa; S. Cooke, Marcus; Richling, Elke; Laffón, Blanca; Valdiglesias, Vanessa; Başaran, Nurşen; Del Bo’, Cristian; Žegura, Bojana; Novak, Matjaž; Stopper, Helga; Vodička, Pavel; Vodenková, Soňa; Moraes de Andrade, Vanessa; Šrámková, Monika; Gábelová, Alena; Collins, Andrew; A. S. Langie, Sabine

doi:10.1038/s41596-020-0398-1

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Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results

Published in Nature Protocols • Oct 26, 2020

Authors:

Peter Möller

Amaya Azqueta

Élisa Boutet-Robinet

Abstract

The comet assay is a widely used test for the detection of DNA damage and repair activity. However, there are interlaboratory differences in reported levels of baseline and induced damage in the same experimental systems. These differences may be attributed to protocol differences, although it is di...

Subject

Comet assay

Comet

Protocol (science)

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