The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice

Yesbolatova, Aisha; Saito, Yuichiro; Kitamoto, Naomi; Makino‐Itou, Hatsune; Ajima, Rieko; Nakano, Risako; Nakaoka, Hirofumi; Fukui, Kosuke; Gamo, Kanae; Tominari, Yusuke; Takeuchi, Haruki; Saga, Yumiko; Hayashi, Ken‐ichiro; T. Kanemaki, Masato

doi:10.1038/s41467-020-19532-z

Public

The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice

Published in Nature Communications • Nov 11, 2020

Authors:

Aisha Yesbolatova

Yuichiro Saito

Naomi Kitamoto

Abstract

Protein knockdown using the auxin-inducible degron (AID) technology is useful to study protein function in living cells because it induces rapid depletion, which makes it possible to observe an immediate phenotype. However, the current AID system has two major drawbacks: leaky degradation and the re...

Subject

Degron

Gene knockdown

Mutant

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