Cre-Controlled CRISPR mutagenesis provides fast and easy conditional gene inactivation in zebrafish

Hans, Stefan; Zöller, Daniela; Hammer, Juliane; Stucke, Johanna; Spieß, Sandra; Kesavan, Gokul; Kroehne, Volker; Sebastián Eguiguren, Juan; Ezhkova, Diana; Petzold, A.; Dahl, Andreas; Brand, Michael

doi:10.1038/s41467-021-21427-6

Public

Cre-Controlled CRISPR mutagenesis provides fast and easy conditional gene inactivation in zebrafish

Published in Nature Communications • Feb 18, 2021

Authors:

Stefan Hans

Daniela Zöller

Juliane Hammer

Abstract

Conditional gene inactivation is a powerful tool to determine gene function when constitutive mutations result in detrimental effects. The most commonly used technique to achieve conditional gene inactivation employs the Cre/loxP system and its ability to delete DNA sequences flanked by two loxP sit...

Subject

CRISPR

Mutagenesis

Cas9

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