Minimizing errors in RT-PCR detection and quantification of SARS-CoV-2 RNA for wastewater surveillance

Ahmed, Warish; L. Simpson, Stuart; M. Bertsch, Paul; Bibby, Kyle; Bivins, Aaron; L. Blackall, Linda; Bofill-Mas, Sílvia; Bosch, Albert; Brandão, João; M. Choi, Phil; Ciesielski, Mark; Donner, Erica; Dsouza, Nishita; H. Farnleitner, Andreas; Gerrity, Daniel; González, Raúl; F. Griffith, John; Gyawali, Pradip; N. Haas, Charles; A. Hamilton, Kerry; Chanditha Hapuarachchi, Hapuarachchige; J. Harwood, Valerie; Haque, Rehnuma; Jackson, Greg; J. Khan, Stuart; Khan, Wesaal; Kitajima, Masaaki; Korajkic, Asja; La Rosa, Giuseppina; A. Layton, Blythe; K. Lipp, Erin; L. McLellan, Sandra; R. McMinn, Brian; Medema, Gertjan; Metcalfe, Suzanne; G. Meijer, Wim; F. Mueller, Jochen; Murphy, Heather; C. Naughton, Coleen; T. Noble, Rachel; Payyappat, Sudhi; Petterson, Susan; Pitkänen, Tarja; Beatriz Rajal, Verónica; Reyneke, Brandon; A. Roman, Fernando; B. Rose, Joan; Rusiñol, Marta; J. Sadowsky, Michael; Sala‐Comorera, Laura; Xiang Setoh, Yin; P. Sherchan, Samendra; Sirikanchana, Kwanrawee; Smith, Wendy; A. Steele, Joshua; Sabburg, Rosalie; M. Symonds, Erin; K. Thai, Phong; V. Thomas, Kevin; Tynan, Josh; Toze, S.; R. Thompson, Janelle; S. Whiteley, Andy; Chui Ching Wong, Judith; Sano, Daisuke; Wuertz, Stefan; Xagoraraki, Irene; Zhang, Qian; G. Zimmer-Faust, Amity; C. Shanks, Orin

doi:10.1016/j.scitotenv.2021.149877

Public

Minimizing errors in RT-PCR detection and quantification of SARS-CoV-2 RNA for wastewater surveillance

Published in The Science of The Total Environment • Aug 25, 2021

Authors:

Warish Ahmed

Stuart L. Simpson

Paul M. Bertsch

Abstract

Wastewater surveillance for pathogens using reverse transcription-polymerase chain reaction (RT-PCR) is an effective and resource-efficient tool for gathering community-level public health information, including the incidence of coronavirus disease-19 (COVID-19). Surveillance of Severe Acute Respira...

Subject

Wastewater

Infection control

Virology

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