Single-molecule localization microscopy

Lelek, Mickaël; Theoni Gyparaki, Melina; Beliu, Gerti; Schueder, Florian; Griffié, Juliette; Manley, Suliana; Jungmann, Ralf; Sauer, Markus; Lakadamyali, Melike; Zimmer, Christophe

doi:10.1038/s43586-021-00038-x

Public

Single-molecule localization microscopy

Published in Nature Reviews Methods Primers • Jun 3, 2021

Authors:

Mickaël Lelek

Melina Theoni Gyparaki

Gerti Beliu

Abstract

Single-molecule localization microscopy (SMLM) describes a family of powerful imaging techniques that dramatically improve spatial resolution over standard, diffraction-limited microscopy techniques and can image biological structures at the molecular scale. In SMLM, individual fluorescent molecules...

Subject

Resolution (logic)

Computer science

Superresolution

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