Quantification of protein cargo loading into engineered extracellular vesicles at single‐vesicle and single‐molecule resolution

Silva, Andreia; Lázaro‐Ibáñez, Elisa; Gunnarsson, Anders; Dhande, Aditya; G. Daaboul, George; Peacock, Ben; Osteikoetxea, Xabier; Salmond, Nikki; Pagh Friis, Kristina; Shatnyeva, Olga; Dekker, Niek

doi:10.1002/jev2.12130

Public

Quantification of protein cargo loading into engineered extracellular vesicles at single‐vesicle and single‐molecule resolution

Published in Journal of Extracellular Vesicles • Aug 1, 2021

Authors:

Andreia Silva

Elisa Lázaro‐Ibáñez

Anders Gunnarsson

Abstract

Abstract Extracellular Vesicles (EVs) have been intensively explored for therapeutic delivery of proteins. However, methods to quantify cargo proteins loaded into engineered EVs are lacking. Here, we describe a workflow for EV analysis at the single‐vesicle and single‐molecule level to accurately qu...

Subject

Extracellular vesicle

Green fluorescent protein

Tetraspanin

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